Cell Types

This page provides lists of the cell types currently being used to create data at the LINCS centers. These tables will be updated as cell types are added.

Broad LINCS Cell Types

The cell types being used by the Broad LINCS team include:

ID Name Status Cell Type Description ATCC # Growth Properties Organ Disease Receptors
1 MCF7 In production Cancer cell line Breast HTB-22 adherent mammary gland; breast adenocarcinoma estrogen receptor, expressed
2 PC3 In production Cancer cell line Prostate CRL-1435 adherent prostate adenocarcinoma vitronectin
3 A549 In production Cancer cell line Lung CCL-185 adherent lung NSCLC
4 A375 In production Cancer cell line Melanoma CRL-1619 adherent skin malignant melanoma
5 HepG2 In production Cancer cell line Liver carcinoma HB-8065 adherent liver hepatocellular carcinoma insulin-like growth factor II (IGF II)
6 VCaP In production Cancer cell line Prostate CRL-2876
7 HCC515 In production Cancer cell line Lung adherent lung NSCLC
8 HT29 In production Cancer cell line Colon HTB-38 adherent colon colorectal adenocarcinoma human adrenergic alpha2A; urokinase receptor (u-PAR), vitamin D (moderate expression)
9 HA1E In production Immortalized normal Kidney epithelial
10 ASC* Passed assay devt
In lysate production		 Primary adipocyte
11 SKL* Passed assay devt
In lysate production		 Primary skeletal myocyte (LONZA)  
12 PHH* Passed assay devt
In lysate production		 Primary hepatocyte adherent
13 NPC* Passed assay devt
In lysate production		 Primary iPS-derived neural progenitor
14 NEU* Passed assay devt
In lysate production		 Primary terminally differentiated neuron
15 MAC* In assay development Primary PBMC-derived macrophages
16 HME1* In assay development Immortalized normal mammary epithelium CRL-4010
17 HF* In assay development Primary fibroblast (epithelial) reduction mammoplasty; normal hTERT immortalized

*nominated by the scientific community

 

HMS LINCS Cell Types

Cell types profiled jointly by the Broad LINCS team and the HMS LINCS team include:

Name Description ATCC # Growth Properties Organ Disease Receptors
MCF7 Breast HTB-22 adherent mammary gland; breast adenocarcinoma estrogen receptor, expressed
MCF10A Breast CRL-10317 adherent mammary gland; breast fibrocystic disease
SKBR3 Breast HTB-30 adherent mammary gland; breast adenocarcinoma
MDAMB231 Breast HTB-26 adherent mammary gland; breast adenocarcinoma epidermal growth factor (EGF), expressed; transforming growth factor alpha (TGF alpha), expressed
BT20 Breast HTB-19 adherent mammary gland; breast carcinoma epidermal growth factor (EGF), expressed; transforming growth factor alpha (TGF alpha), expressed

 

Experimental Verification

The teams will mainly use genotypes obtained from SNP arrays for comparison to in CCLE and with the Sanger SNP60 CGP dataset, as well as a panel of 48 SNPs to fingerprint the cell types.

For more information on the guidance being used by the LINCS teams for experimental verification, see the following article: American Type Culture Collection Standards Development Organization Workgroup ASN-0002. Cell line misidentification: the beginning of the end. Nat Rev Cancer. 2010;10:441-448. Epub 2010 May 7.